背景資料

Reactive oxygen species （ROS） are chemically reactive molecules containing oxygen。 Examples include superoxide， hydroxyl radical， singlet oxygen and peroxides。 ROS is highly reactive due to the presence of unpaired valence shell electrons。 ROS forms as a natural byproduct of the normal metabolism of oxygen and have important roles in cell signaling and homeostasis。 However， during times of environmental stress （e。g。， UV or heat exposure）， ROS levels can increase dramatically。 This may result in significant damage to cell structures。 Cumulatively， this is known as oxidative stress。 ROS are also generated by exogenous sources such as ionizing radiation。 Under conditions of oxidative stress， ROS production is dramatically increased， resulting in subsequent alteration of membrane lipids， proteins， and nucleic acids。 Oxidative damage of these biomolecules is associated with aging as well as with a variety of pathological events， including atherosclerosis， carcinogenesis， ischemic reperfusion injury， and neurodegenerative disorders。 ROS Brite HPF is a fluorogenic probe to measure hydroxyly radical in cells using conventional fluorescence microscopy， high-content imaging， microplate fluorometry， or flow cytometry。 The cell-permeant ROS Brite HPF reagent is nonfluorescent and produces bright green fluorescence upon reaction with hydroxyl radical。 The resulting fluorescence can be measured using fluorescence imaging， high-content imaging， microplate fluorometry， or flow cytometry。 In the presence of peroxidase， HPF also reacts with hydrogen peroxide。 HPF has good selectivity to hydroxyl radical compared to other ROS （e。g。， supoeroxide and nitric oxide）。 APF and HPF show relatively high resistance to light-induced oxidation。 APF and HPF are nonfluorescent until they react with the hydroxyl radical or peroxynitrite anion。

產品描述

ROS Brite HPF產品的光譜學相關參數為Ex（nm）=492/ Em（nm）515；其對應分子量大小為424。4。

產品形式

提供的ROS Brite HPF產品為固體形式，我們建議將其溶解到DMSO溶液中使用或保存。

保存建議

對于ROS Brite HPF產品，我們推薦使用常溫進行運輸。在您收到貨物后，我們建議在低于-15℃溫度下冷凍保存，要求儲存環境干燥并避免光照。產品有效期為12個月。

其他

美國AAT Bioquest Inc。（前身是ABD Bioquest，Inc。）是一家為從事生命科學研究、診斷研發及藥物開發的科學家研發、生產和銷售生物分析研究試劑和試劑盒的公司。公司致力于光譜學檢測領域，包括顯色、熒光和生物發光技術。AAT Bioquest的產品幫助的科學家和生物醫藥研究者更好的了解生物化學，免疫學，細胞生物學和分子生物學等領域。作為AAT Bioquest Inc。的中國區域代理，艾美捷科技為中國客戶提供光譜學檢測技術并應用于生化、生理代謝和細胞分析領域的產品，包括顯色，熒光和發光技術等全系列解決方案。