背景資料

Recombinant Human Sonic Hedgehog is part of a small group of secreted proteins that are vital for development in both vertebrates and invertebrates. 3 mammalian hedgehog genes （sonic，desert，Indian） share about 60% homology. The Human Sonic Hedgehog is 99% homologous to the mouse gene. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube，the anterior-posterior limb axis，and the ventral somites. Sonic HedgeHog binds to the patched receptor，which functions in association with smoothened，to activate the transcription of target genes. In the absence of sonic HedgeHog，patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor，the gli oncogene. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites，and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog exhibits both floor plate- and motor neuron-inducing activity. Mutations in a long-range Sonic HedgeHog enhancer located in an intron of the limb region 1 gene result in preaxial polydactyly.

序列

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來(lái)源宿主

大腸桿菌中重組表達(dá)

溶解建議

提供的人音猬因子（SHH）產(chǎn)品為過(guò)濾滅菌的白色凍干粉末；該凍干粉末是從含1mg/ml人音猬因子（SHH）的10mM 磷酸鈉緩沖液（pH 7.5）中凍干產(chǎn)生的.我們建議加無(wú)菌的水以配制工作儲(chǔ)存液到0.1-0.5mg/ml，后繼實(shí)驗(yàn)過(guò)程中可根據(jù)實(shí)驗(yàn)要求進(jìn)一步稀釋.

保存建議

人音猬因子（SHH）在室溫可保存3周，或置于-18℃凍存用于長(zhǎng)期保存.重新溶解后可以在4℃冷藏保存至少2-7天；如果想延長(zhǎng)保存時(shí)間，建議分裝后置于-18℃凍存.請(qǐng)避免反復(fù)凍融.

其他

ProSpec 是一家全球著名細(xì)胞因子蛋白生產(chǎn)和研發(fā)的公司，具有20多年的生產(chǎn)經(jīng)驗(yàn)，專注于蛋白（重組及合成）生產(chǎn)研發(fā)。我們的核心產(chǎn)品包括：干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長(zhǎng)因子、瘦素、干細(xì)胞因子、催乳素、表皮生長(zhǎng)因子、胰島素樣生長(zhǎng)因子、神經(jīng)生長(zhǎng)因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。 的生產(chǎn)工藝和規(guī)模使其可以提供毫克到克級(jí)蛋白，價(jià)格優(yōu)于同類公司。絕大部分產(chǎn)品是天然成熟型蛋白，而不含有標(biāo)簽蛋白。考慮到大部分研究者希望能更靈活地配制蛋白溶液，ProSpec-Tany絕大部分產(chǎn)品沒(méi)有添加保護(hù)劑或鹽，這就對(duì)工藝提出了更高的要求。同時(shí)，ProSpec-Tany絕大部分產(chǎn)品為凍干粉，因此易于運(yùn)輸和保存。