背景資料

Matrix metalloproteinases are a family of zinc and calcium-dependent endopeptidases that break down extracellular matrix proteins. The MMP9 is secreted as a 92kDa zymogen. Cleavage of ProMMP-9 results in the active enzyme， having a molecular weight of approximately 82kDa. MMP9 is composed of the following domains: a gelatin-binding domain consisting of three fibronectin type II units， a catalytic domain containing the zinc-binding site， a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by the several cell types: monocytes， macrophages， neutrophils， keratinocytes， fibroblasts， osteoclasts and endothelial cells. MMP9 is involved in inflammatory responses， tissue remodeling， wound healing， tumor growth and metastasis. MMP9 may also play an important part in local proteolysis of the extracellular matrix and in leukocyte migration， as well as in bone osteoclastic resorption. MMP9 cleaves type IV and type V collagens into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. MMP9 can also degrade fibronectin but not laminin or Pz-peptide.

MMP9 defects may be a cause of susceptibility to intervertebral disc disease（IDD)， also known as lumbar disk herniation（LDH).

序列

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來源宿主

大腸桿菌中重組表達

溶解建議

提供的人基質金屬蛋白酶-9（MMP 9）產品為過濾滅菌的透明溶液；其中人基質金屬蛋白酶-9（MMP 9）產品溶解于20mM Tris-HCl pH 8.0，50%甘油溶液中.

保存建議

人基質金屬蛋白酶-9（MMP 9）溶液可以在4℃冷藏保存2-4周；如果想延長保存時間，建議分裝后置于-20℃凍存.請避免反復凍融.

其他

ProSpec 是一家全球著名細胞因子蛋白生產和研發的公司，具有20多年的生產經驗，專注于蛋白（重組及合成）生產研發。我們的核心產品包括：干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長因子、瘦素、干細胞因子、催乳素、表皮生長因子、胰島素樣生長因子、神經生長因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。 的生產工藝和規模使其可以提供毫克到克級蛋白，價格優于同類公司。絕大部分產品是天然成熟型蛋白，而不含有標簽蛋白。考慮到大部分研究者希望能更靈活地配制蛋白溶液，ProSpec-Tany絕大部分產品沒有添加保護劑或鹽，這就對工藝提出了更高的要求。同時，ProSpec-Tany絕大部分產品為凍干粉，因此易于運輸和保存。