Product Details

The RMP1-14 monoclonal antibody reacts with mouse PD-1 (programmed death-1) also known as CD279. PD-1 is a 50-55 kDa cell surface receptor encoded by the Pdcd1 gene that belongs to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. PD-1 expression declines after successful elimination of antigen. Additionally, Pdcd1 mRNA is expressed in developing B lymphocytes during the pro-B-cell stage. PD-1’s structure includes a ITIM (immunoreceptor tyrosine-based inhibitory motif) suggesting that PD-1 negatively regulates TCR signals. PD-1 signals via binding its two ligands, PD-L1 and PD-L2 both members of the B7 family. Upon ligand binding, PD-1 signaling inhibits T-cell activation, leading to reduced proliferation, cytokine production, and T-cell death. Additionally, PD-1 is known to play key roles in peripheral tolerance and prevention of autoimmune disease in mice as PD-1 knockout animals show dilated cardiomyopathy, splenomegaly, and loss of peripheral tolerance. Induced PD-L1 expression is common in many tumors including squamous cell carcinoma, colon adenocarcinoma, and breast adenocarcinoma. PD-L1 overexpression results in increased resistance of tumor cells to CD8 T cell mediated lysis. In mouse models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 and its receptor PD-1. For these reasons anti-PD-1 mediated immunotherapies are currently being explored as cancer treatments. Like the J43 antibody the RMP1-14 antibody has been shown to block the binding of both mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1.

RMP1-14單克隆抗體與小鼠PD-1（程序性死亡-1）反應，也稱為CD279。PD-1是由Pdcd50基因編碼的55-1 kDa細胞表面受體，屬于Ig超家族的CD28家族。PD-1在CD4和CD8胸腺細胞以及活化的T和B淋巴細胞和骨髓細胞上短暫表達。PD-1在成功消除抗原后表達下降。此外，Pdcd1 mRNA在前B細胞階段的發育B淋巴細胞中表達。PD-1的結構包括ITIM（基于免疫受體酪-氨-酸的抑制基序），表明PD-1負調節TCR信號。PD-1通過結合其兩個配體PD-L1和PD-L2來發出信號，PD-L7和PD-L1都是B1家族的成員。配體結合后，PD-1信號傳導抑制T細胞活化，導致增殖減少，細胞因子產生和T細胞死亡。

此外，已知PD-1在小鼠外周耐受和預防自身免疫性疾病中起關鍵作用，因為PD-1基因敲除動物表現出擴張型心肌病，脾腫大和外周耐受性喪失。誘導PD-L8表達在許多腫瘤中很常見，包括鱗狀細胞癌、結腸腺癌和乳腺腺癌。PD-L1過表達導致腫瘤細胞對CD1 T細胞介導的裂解的耐藥性增加。在黑色素瘤小鼠模型中，腫瘤生長可以通過阻斷PD-L1與其受體PD-43之間相互作用的抗體治療來暫時阻止。由于這些原因，目前正在探索抗PD-1介導的免疫療法作為癌癥治療方法。與J14抗體一樣，RMP1-2抗體已被證明可以阻斷小鼠PD-L1-Ig和小鼠PD-L<>-Ig與PD-<>的結合。

艾美捷體內小鼠PD-1抗體#BE0146詳細信息：

同種型 大鼠IgG2a， κ

推薦的同種型對照 體內MAb大鼠IgG2a同種型對照，抗三-硝-基-苯-酚

推薦的稀釋緩沖液 體內純 pH 7.0 稀釋緩沖液

免疫原 用小鼠PD-1 cDNA轉染的敘利亞倉鼠BKH細胞

應用程序 PD-1/PD-L信號傳導的體內阻斷

配方 PBS，pH 7.0

不含穩定劑或防腐劑

內毒素 <2EU/mg （<0.002EU/μg）

通過LAL凝膠凝血測定法測定

純度 >95%

由 SDS-PAGE 決定

無 菌 0.2 μM 過濾

生產 從無動物設施中的組織培養上清液中純化

純化 蛋白 G

里德 AB_10949053

分子量 150 千達

存儲 抗體溶液應以4°C的儲備濃度儲存。 不要凍結。

體內小鼠PD-1抗體部分文獻參考：

in vivo blocking of PD-1/PD-L signaling

Ngiow, S. F., et al. (2015). "A Threshold Level of Intratumor CD8+ T-cell PD1 Expression Dictates Therapeutic Response to Anti-PD1" Cancer Res 75(18): 3800-3811.

in vivo blocking of PD-1/PD-L signaling

Evans, E. E., et al. (2015). "Antibody Blockade of Semaphorin 4D Promotes Immune Infiltration into Tumor and Enhances Response to Other Immunomodulatory Therapies" Cancer Immunol Res 3(6): 689-701.

in vivo blocking of PD-1/PD-L signaling

Zelenay, S., et al. (2015). "Cyclooxygenase-Dependent Tumor Growth through Evasion of Immunity" Cell 162(6): 1257-1270.