The 11B11 monoclonal antibody reacts with mouse IL-4 (interleukin-4) a multifunctional 14 kDa cytokine. IL-4 is expressed primarily by activated Th2 cells and NK cells, and at lower levels by mast cells, and basophils. IL-4 signals through the IL-4Rα. Upon receptor binding IL-4 stimulates activated B and T lymphocyte proliferation, and the differentiation of B cells into plasma cells. It also induces B cell class switching to IgE, and up-regulates MHC class II production while decreasing the production of Th1 cells, macrophages, IFNγ, and dendritic cell IL-12. Like other Th2 associated cytokines, IL-4 is involved in the airway inflammation observed in the lungs of patients with allergic asthma. The 11B11 monoclonal antibody has been shown to neutralize the bioactivity of natural or recombinant IL-4.

Isotype Rat IgG1, κ

Recommended Isotype Control(s) InVivoMAb rat IgG1 isotype control, anti-horseradish peroxidase

Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer

Immunogen Partially purified native mouse IL-4

Reported Applications in vivo IL-4 neutralization

in vitro IL-4 neutralization

in vivo IL-4 receptor stimulation (as a complex with IL-4)

Flow cytometry

Western blot

Formulation PBS, pH 7.0

Contains no stabilizers or preservatives

Endotoxin <2EU/mg (<0.002EU/μg)

Determined by LAL gel clotting assay

Purity >95%

Determined by SDS-PAGE

Sterility 0.2 μM filtered

Production Purified from tissue culture supernatant in an animal free facility

Purification Protein G

RRID AB_1107707

Molecular Weight 150 kDa

Storage The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

11B11單克隆抗體與小鼠IL-4（白細(xì)胞介素-4）反應(yīng)，這是一種多功能14kDa細(xì)胞因子。IL-4主要由活化的Th2細(xì)胞和NK細(xì)胞表達(dá)，在較低水平上由肥大細(xì)胞和嗜堿性粒細(xì)胞表達(dá)。IL-4通過(guò)IL-4Rα發(fā)出信號(hào)。受體結(jié)合后，IL-4刺激活化的B和T淋巴細(xì)胞增殖，以及B細(xì)胞分化為漿細(xì)胞。它還誘導(dǎo)B細(xì)胞類(lèi)切換到IgE，并上調(diào)MHC II類(lèi)的產(chǎn)生，同時(shí)減少Th1細(xì)胞，巨噬細(xì)胞，IFNγ和樹(shù)突狀細(xì)胞IL-12的產(chǎn)生。與其他Th2相關(guān)細(xì)胞因子一樣，IL-4參與過(guò)敏性哮喘患者肺部觀察到的氣道炎癥。11B11單克隆抗體已被證明可以中和天然或重組IL-4的生物活性。

艾美捷InVivoMAb 抗小鼠 IL-4#BE0045基本參數(shù)：

同種型 大鼠IgG1， κ

推薦的同種型對(duì)照 體內(nèi)MAb大鼠IgG1同種型對(duì)照，抗辣根過(guò)氧化物酶

推薦的稀釋緩沖液 體內(nèi)純 pH 7.0 稀釋緩沖液

免疫原 部分純化的天然小鼠IL-4

報(bào)告的應(yīng)用程序 體內(nèi)IL-4體外中和IL-4體內(nèi)中

和

IL-4受體刺激（與IL-4復(fù)合）

流式細(xì)胞術(shù)

蛋白質(zhì)印跡

配方 PBS，pH 7.0

不含穩(wěn)定劑或防腐劑

內(nèi)毒素 <2EU/mg （<0.002EU/μg）

通過(guò)LAL凝膠凝血測(cè)定法測(cè)定

純度 >95%

由 SDS-PAGE 決定

無(wú) 菌 0.2 μM 過(guò)濾

生產(chǎn) 從無(wú)動(dòng)物設(shè)施中的組織培養(yǎng)上清液中純化

純化 蛋白 G

里德 AB_1107707

分子量 150 千達(dá)

存儲(chǔ) 抗體溶液應(yīng)以4°C的儲(chǔ)備濃度儲(chǔ)存。 不要凍結(jié)。

InVivoMAb抗小鼠IL-4部分文獻(xiàn)引用：

in vitro IL-4 neutralization

Hill, E. V., et al. (2015). "Glycogen synthase kinase-3 controls IL-10 expression in CD4(+) effector T-cell subsets through epigenetic modification of the IL-10 promoter" Eur J Immunol 45(4): 1103-1115.

in vitro IL-4 neutralization

Choi, Y. S., et al. (2015). "LEF-1 and TCF-1 orchestrate TFH differentiation by regulating differentiation circuits upstream of the transcriptional repressor Bcl6" Nat Immunol 16(9): 980-990.

in vitro IL-4 neutralization

Kim, Y. U., et al. (2015). "Regulation of autoimmune germinal center reactions in lupus-prone BXD2 mice by follicular helper T cells" PLoS One 10(3): e0120294.

in vitro IL-4 neutralization

Hou, L., et al. (2015). "The protease cathepsin L regulates Th17 cell differentiation" J Autoimmun. S 0896-8411(15): 30024-X.