Synthesis and structure

Atto™-dextrans are synthesised from well-characterized dextran fractions derived from Leuconostoc mesenteroides via the succinimidyl-derivative of Atto488. After purification from non-bound dye, the products are controlled for Mw, solubility, DS, free dye and loss on drying.

The products are designated by the approximate molecular weights of the dextran fractions used. Thus, for example, the product Atto-dextran 4 has a molecular weight of approx. 4000. The actual molecular weight is determined by GPC. This value is supplied with the Certificate of Analysis. The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear α-(1-6)-linked

glucose chain with however a low percentage (2-5%) of α-(1-3) branches distributed along the chain. The dextran fractions used are from Mw of 4000 to 150000 and are carefully controlled by GPC, optical rotation, absorbance and other control parameters.

ATTO488-葡聚糖的結(jié)構(gòu)特征

Spectral properties

Atto488-dextran has an absorbance maximum at 502 nm (in borate buffer, pH 9.0) and an emission wavelength of 524 nm. The Atto-dye has a somewhat higher quantum yield than fluorescein and displays a better photo-stability. The DS lies between 0.001-0.008.

Applications

Atto488-dextrans are primarily used for studying permeability and transport in cells and tissues. An added bene?fit is that measurements of the fluorescence provide quantitative data on the permeability of healthy and diseased tissues. Such studies can be performed in real time by intravital fluorescence microscopy. The technique offers high sensitivity and concentrations down to 1μg/ml can be detected in tissue fluids

艾美捷ATTO488-葡聚糖#AT488D4/AT488LD10, 以橙色粉末形式提供，易溶于水。ATTO488-葡聚糖主要用于研究細(xì)胞和組織中的滲透性和轉(zhuǎn)運(yùn)。

ATTO488-葡聚糖是由來(lái)源于明串珠菌的具有良好特征的右旋糖聚糖組分合成的通過(guò)Atto488的琥珀酰亞胺衍生物形成的腸系膜。從非結(jié)合染料中純化后控制產(chǎn)品的Mw、溶解度、DS、游離染料和干燥損失。產(chǎn)品由所用葡聚糖部分的近似分子量指-定。因此，例如，產(chǎn)物Atto-右旋糖酐4具有約4000的分子量。實(shí)際分子量通過(guò)GPC測(cè)定。此值隨分析證書一起提供。這個(gè)所用的葡聚糖來(lái)自間質(zhì)明串珠菌B-512F，其本質(zhì)上是線性α-（1-6）連接的然而，沿鏈分布的α-（1-3）分支的百分比較低（2-5%）。所使用的葡聚糖級(jí)分的Mw為4000至150000，并通過(guò)GPC、光學(xué)轉(zhuǎn)速、吸光度和其他控制參數(shù)。

物理特性：

Atto-葡聚糖是一種橙色粉末，可自由溶解于水或電解質(zhì)溶液中。這個(gè)由于存在磺酸鹽和氨基部分。Atto-葡聚糖不溶于大多數(shù)有機(jī)溶劑，丙酮、氯仿、乙酸乙酯等。

應(yīng)用：

Atto488葡聚糖主要用于研究細(xì)胞和組織中的滲透性和轉(zhuǎn)運(yùn)。一額外的好處是熒光測(cè)量提供了關(guān)于健康和患病組織的滲透性。這種研究可以通過(guò)活體內(nèi)注射實(shí)時(shí)進(jìn)行熒光顯微鏡。該技術(shù)具有高靈敏度，濃度低至1μg/ml可以在組織液中檢測(cè)到。

相關(guān)研究：

AT488LD10 ATTO488-lysine-dextran 10 10000 5mg

AT647NLD70 ATTO647N-lysine-dextran 70000 5mg