培養基和添加劑 | IMDM培養基(GIBCO,貨號12200036,添加0.005 mg/ml insulin, 0.005 mg/ml transferrin 和 5 ng/ml GM-CSF,),95%;優質胎牛血清,5%。 氣相:空氣,95%;二氧化碳,5%。 溫度:37攝氏度。 生長條件: 這株細胞的*生長依賴GM-CSF。 10%濃度的U-87MG (ATCC HTB-14)條件培養基可以代替培養基中的GM-CSF(見J. Immunol. 139:3348- 3354, 1987)。 |
REFERENCE | Lange B, et al. Growth factor requirements of childhood acute leukemia: establishment of GM-CSF-dependent cell lines. Blood 70: 192-199, 1987. PubMed: 3496132 Santoli D, et al. Synergistic and antagonistic effects of recombinant human interleukin (IL) 3, IL-1 alpha, granulocyte and macrophage colony-stimulating factors (G-CSF and M-CSF) on the growth of GM-CSF-dependent leukemic cell lines. J. Immunol. 139: 3348-3354, 1987. PubMed: 3500218 |