HPAC細胞傳代細胞,活性好、存活率高、質量保證,生長狀態良好,沒有細菌 真菌 支原體等微生物污染。HPAC細胞一般以T25培養瓶包裝,容積75ml細胞數量可達10的6次方左右。
細胞名稱:人胰腺腺泡上皮癌細胞;HPAC
規格:T25培養瓶,1×106cells
形態特征:上皮細胞樣
生長特性:貼壁
產品描述
培養條件: MDEM 10%FBS
傳代方法: 1:3-1:6; 3~5天換液1次。
STR位點信息:
STR Profile | AMEL | CSF1PO | D13S317 | D16S539 | D5S818 | D7S820 | TH01 | TPOX | vWA |
HPAC | X | 13 | 11 | 9,10 | 12 | 10,12 | 9,3 | 10,11 | 15,17 |
HPAC細胞復蘇時如何換液呢?
1凍存細胞取出后37℃速溶,取15ml離心管,加入10ml含血清培養基,將凍存管中液體(通常1.5ml)也加入離心管中,習慣輕吹幾下混勻,1200轉常溫離心5min,去掉上清,加入5ml含血清培養基,輕吹制成細胞懸液,加入到培養瓶中,即可。
注意,新復蘇的HPAC細胞不要經常去看去動。
換液的話,只要把培養基吸出,再加入新的培養基就可以了
如果你復蘇的細胞長得很不均勻,可以*消化下來再混勻后在重新加進去(像正常細胞一樣做)。
兩種方案可供選擇:
一、復蘇時,行離心,棄上清后,種入瓶中。主要目的是防止DMSO對細胞造成損害,但剛復蘇的細胞教脆弱,離心易導致細胞破碎。
二、HPAC細胞復蘇時,直接將凍存管里的液體倒入培養瓶中,另外添加適量的培養基,孵箱24h,待貼壁后行常規換液。省去了離心一步,避免離心時細胞破裂。但DMSO未能去除,長時間培養可能會對細胞有損害。個人覺得第二種方案較方便。
Classical Media
09011 MEM MEM MEM with Earle's Salts and L-Glutamine 500 ml
09021 MEM MEM MEM with Hank's Salts and L-Glutamine 500 ml
09031 MEM MEM MEM with Eagles's Salts and Glutamine 500 ml
09111 M199E M199E Medium 199 with Earle's Salts and L-Glutamine 500 ml
09121 M199H M199H Medium 199 with Hanks Salts and L-Glutamine 500 ml
09211 DMEM DMEM DMEM with L-Glutamine and Sodium Pyruvate 500 ml
09221 DMEM DMEM DMEM with High-Glucose - L-Glutamine and Sodium Pyruvate 500 ml
09221-1L DMEM DMEM DMEM with High-Glucose - L-Glutamine and Sodium Pyruvate 1L
09231 DMEM DMEM DMEM with L-Glutamine - Sodium Pyruvate and 25 mM HEPES 500 ml
09241 DMEM DMEM DMEM with High-Glucose - L-Glutamine - Sodium Pyruvate and 25 mM HEPES 500 ml
09251 -1L DMEM-prf DMEM-prf DMEM with High Glucose - L-Glutamine and Sodium Pyruvate - phenol red-free 1L
09311 Ham's F-10 Ham's F-10 F-10 with L-Glutamine and 25 mM HEPES 500 ml
09321 Ham's F-12 Ham's F-12 F-12 with L-Glutamine and 25 mM HEPES 500 ml
09411 DMEM/F-12 DMEM/F-12 DMEM/F-12 with L-Glutamine 500 ml
09421 DMEM/F-12 DMEM/F-12 DMEM/F-12 with L-Glutamine and 15 mM HEPES 500 ml