小鼠海馬神經膠質細胞(EGFP標記)腸平滑肌細胞 Many types of cells
腸微血管內皮細胞 Many types of cells
腸粘膜上皮細胞 Many types of cells
腸粘膜上皮細胞 Many types of cells
腸粘膜上皮細胞 Many types of cells
小鼠海馬神經膠質細胞(EGFP標記)材料和試劑
1. 細胞:貼壁細胞株
2. 試劑:0.25%*、1640培養基(含10%小牛血清)
3. 儀器和器材:倒置顯微鏡,培養箱、培養瓶、吸管、廢液缸等
細胞接種或傳代以后,實驗者每天或至多間隔1~2d,要對細胞做常規性檢查。觀察細胞形態和生長情況以及培養的pH變化、有無污染等。根據細胞動態變化,做換液或傳代處理,如發現異常情況應及時采取措施。
技術步驟
①將對數生*的細胞濃度調整為1~10×104細胞/ml,按103~104個細胞接種于96孔板,每孔100 μl;
②培養細胞24小時后,對其進行不同處理,每個處理設三個平行對照;
③(MTT法):適當培養時間后,取出細胞,倒掉培養液,每孔加入5 mg/ml新鮮配制的MTT溶液,溫育4小時,再次倒掉上清液,每孔加入200ml DMSO,搖勻后,酶標儀檢測吸光值。
PTEN/MMAC1 一種腫瘤抑制基因抗原 0.5mg
PT141(Bremelanotide PT-141 1g
PMP2/FABP8(peripheral myelin protein 2 0.5mg
PLGF (Placenta growth factor 0.5mg
PLGF (Placenta growth factor 0.5mg
PLGF (Placenta growth factor fragment 0.5mg
PLAU/uPA (Plasminogen activator,urokinase 0.5mg
PKA(Protein Kinase A 0.5mg
PIWIL1(piwi-like 1 0.5mg
PIBF(progesterone induced blocking factor 0.5mg
PI3K 磷脂酰肌醇激酶(抗原 0.5mg
phospho-Tau protein (pThr489 0.5mg
phospho-STAT1(p-Tyr701 0.5mg
phospho-Smad2(p-Ser465 0.5mg
phosphor-SMAD(p-Ser425 0.5mg
Phospho-PAK4 (Ser99 0.5mg
phospho-p38 ERK/MAPK14 (pThr180/pTyr182 0.5mg
phospho-NFKB p65 (pSer536 0.5mg
phospho-KLF5/Krueppel-like factor 5 (pSer272 0.5mg
phospho-Klf5/CKLF/Kruppel like factor 5, Human, mo, rat, horse, bovine, rabbit, pig, dog 磷酸化腸道內富含的Kruppel樣因子5抗原 0.5mg
phospho-JNK1/2/3(pThr183/pTyr185 0.2ml
phospho-HP1/heterochromatin protein 1 (pTyr24 0.5mg
phospho-heterochromatin protein 1 (pTyr41 0.5mg
phospho-GSK-3 Beta(pSer9 0.5mg
phospho-GSK3 Alpha/ Beta(alpha + beta 0.5mg
phospho-FAK(pTyr577 0.5mg
phospho-FAK(pSer732 0.5mg
溫馨提示:若客戶對細胞造成污染;因客戶對不正確操作致細胞狀態不好;非FDCC*細胞培養體系致的細胞狀態不好;發現狀態不好,請在一周內及時,我們將盡力為您解決問題。