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尼古丁唾液金標檢測卡
廣州健侖生物科技?有限公司
本司長期供應尼古丁(可替寧)檢測試劑盒,其主要品牌包括美國NovaBios、廣州健侖、廣州創侖等進口產品,國產產品,試劑盒的實驗方法是膠體金方法。
我司還提供其它進口或國產試劑盒:登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產品。
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【包裝規格】
1人份/袋,40人份/盒
【預期用途】
尼古丁(Nicotine)是煙草中的主要生物堿,是導致吸煙成癮的物質動因,也是評價人體攝入煙草煙霧的常用指標。但因為尼古丁半衰期短,無法作為標志物檢測,其代謝物可替寧因為半衰期長作為吸煙和戒煙的標志物。
本品采用競爭抑制法和膠體金免疫層析技術,用于快速定性檢測人體唾液中的可替寧,適用于評價煙草煙霧攝入的初步篩查。
【主要組成成份】
【檢驗方法】
尼古丁唾液金標檢測卡
3.1.5 糞便
有腸結核可疑時,可采集糞便進行細菌學檢查,患肺結核的牛只,有時在糞便中亦可檢出結核分枝桿菌。因牛常將痰液吞咽至消化道內,隨糞便排出。盡量采集混有粘液或膿血的糞便.
取糞便30g,加15%~20%安替福民溶液15mL和蒸餾水55mL,混合,經2h~3h后,3000r/min~4000r/min離心30min,傾去上清液,加10mL蒸餾水于沉淀物中,將此液用紗布過濾后接種于豚鼠皮下,經1.5~2個月后剖檢。
或將糞便加2倍量滅菌蒸餾水磨碎,然后加氯化鈉至飽和。當液體中殘渣沉淀后(液體*澄清),浮起的薄膜含有大量的結核分枝桿菌。取出薄膜,用等量的4%氫氧化鈉(見附錄A中A2)充分混合,置37℃中3h,然后離心,取沉淀物用8%鹽酸中和后,即可作染色鏡檢、培養和動物試驗。
糞便中如有粘液或膿血,可挑取粘液膿血選用任何一種消化液處理后檢驗。
如系純糞便,取5g~10g,加生理鹽水約3~4倍混合后,用細銅紗網過濾,取濾液置室溫中自然沉淀,然后取上層懸液置于另一大離心管或燒杯內,加消化液約3~4倍量,置37℃溫箱1h~2h,同上法處理后檢驗。
3.1.6 組織細菌
盡量采集有結節病灶的部位。豬易患頸部淋巴結結核,亦易患腸系膜淋巴結核,且常呈鈣化或干酪樣病變。家禽常在腸、脾、肝發生結核病灶,有時亦可見于肺或卵巢。
病變組織需先研磨,制成乳劑,通常取組織乳劑或其他液狀病料2mL~4mL,加入等量的5%氫氧化鈉溶液,充分振搖5min~10min,或搖至發生液化為止,液化后經3000r/min~4000r/min離心15min~30min,沉淀物加1滴酚紅作指示劑,以2mol/L鹽酸中和至淡紅色后,作染色鏡檢、培養和動物試驗。
如病料為膿狀或干酪狀或鈣化的結節病灶組織,可直接作染色鏡檢,往往可以檢出眾多的結核分枝桿菌。但得到陰性結果時,應濃縮處理后檢驗。
3.2 檢驗方法
3.2.1 染色鏡檢
3.2.1.1 涂片
先在玻片上涂布一層薄甘油蛋白(雞蛋白20mL,甘油20mL,水楊酸鈉0.4g,混勻)然后吸取標本滴加其上,涂布均勻。
想了解更多的韓國SD產品及服務請掃描下方二維碼:我司還提供其它進口或國產試劑盒:登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產品。
二維碼掃一掃
【公司名稱】 廣州健侖生物科技有限公司
【】 楊永漢
【】
【騰訊 】
【公司地址】 廣州清華科技園創新基地番禺石樓鎮創啟路63號二期2幢101-3室
【企業文化宣傳】
3.1.5 excrement
There is suspicious bowel tuberculosis can be collected for bacteriological examination of faeces, tuberculosis in cattle, and sometimes in the stool can be detected in Mycobacterium tuberculosis. Due to cow often swallow sputum to the digestive tract, with the excretion. Try to collect mixed with mucus or pus and blood stool.
Take feces 30g, add 15% ~ 20% of Anfetuomin solution 15mL and distilled water 55mL, after 2h ~ 3h, 3000r / min ~ 4000r / min centrifugation 30min, the supernatant was decanted, add 10mL of distilled water in the precipitate , The liquid was filtered with gauze and inoculated guinea pigs subcutaneously, by 1.5 to 2 months after necropsy.
Or excrement plus 2 times the amount of sterile distilled water, then add sodium chloride to saturation. When the residue in the liquid is precipitated (the liquid is compley clear), the floating membrane contains a large amount of Mycobacterium tuberculosis. Remove the membrane, with an equal amount of 4% sodium hydroxide (see Appendix A in A2) mixed thoroughly, set at 37 ℃ for 3h, then centrifuged, the precipitate was neutralized with 8% hydrochloric acid, can be used for staining microscopy, culture And animal experiments.
Any mucus or pus and blood in the stool can pick mucus pus and use any kind of digestive juice test after treatment.
If the pure manure, take 5g ~ 10g, plus about 3 to 4 times the normal saline mixed with fine copper gauze filter, the filtrate was taken at room temperature natural precipitation, and then take the upper suspension placed in another large centrifuge tube or beaker Within the plus digestive juice about 3 to 4 times the amount of 37 ℃ incubator 1h ~ 2h, the same way after the test.
3.1.6 tissue bacteria
Try to collect the site of nodular lesions. Pig susceptible to cervical lymph node tuberculosis, but also susceptible to mesenteric lymph nodes, and often calcification or caseous lesions. Poultry often intestinal, spleen, liver tuberculosis occurs, sometimes seen in the lungs or ovaries.
Lesion tissue to be ground first, made of emulsion, usually take tissue emulsion or other liquid material 2mL ~ 4mL, add the same amount of 5% sodium hydroxide solution, shake 5min ~ 10min, or shake until liquefaction, after liquefaction After 3000r / min ~ 4000r / min centrifugation 15min ~ 30min, the precipitate plus 1 drop of phenol red as an indicator to 2mol / L hydrochloric acid and to reddish, for microscopic examination, culture and animal experiments.
If the material is pus or cheese-like or calcified nodules lesions, can be directly for staining microscopy, often can detect a large number of Mycobacterium tuberculosis. However, negative results should be concentrated test after treatment.
3.2 test method
3.2.1 staining microscopy
3.2.1.1 smear
First, apply a thin layer of thin glycerin (egg white 20mL, glycerin 20mL, sodium salicylate 0.4g, mix well) on the slide and then aspirate the sample drop on it, coating evenly.
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