當前位置:廣州健侖生物科技有限公司>>食品安全檢測>>尼古丁快速檢測試劑盒>> 廣州創侖尼古丁唾液測試紙
尼古丁唾液測試紙 廣州創侖
廣州健侖生物科技?有限公司
本司長期供應尼古丁(可替寧)檢測試劑盒,其主要品牌包括美國NovaBios、廣州健侖、廣州創侖等進口產品,國產產品,試劑盒的實驗方法是膠體金方法。
我司還提供其它進口或國產試劑盒:登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產品。
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【包裝規格】
1人份/袋,40人份/盒
【預期用途】
尼古丁(Nicotine)是煙草中的主要生物堿,是導致吸煙成癮的物質動因,也是評價人體攝入煙草煙霧的常用指標。但因為尼古丁半衰期短,無法作為標志物檢測,其代謝物可替寧因為半衰期長作為吸煙和戒煙的標志物。
本品采用競爭抑制法和膠體金免疫層析技術,用于快速定性檢測人體唾液中的可替寧,適用于評價煙草煙霧攝入的初步篩查。
【主要組成成份】
【檢驗方法】
尼古丁唾液測試紙
因此,可通過縮短引物序列或取消引物序列對核酸庫進行優化。在雙RNA庫篩選方法中,設計的核酸庫的引物序列可自身折疊互補,在引物區形成雙鏈結構,從而使引物序列與靶分子的非特異性格結合可能性格大大降低。適配體 (Aptamer) 是一種經體外篩選技術得到的寡核苷酸序列 (RNA或 DNA),與相應的配體有嚴格的識別能力和高度的親和力,大小一般約6-40 kDa。單鏈寡核苷酸,特別是RNA的一些二級結構,如發夾、莖環、假節、凸環、G-四聚體等,可使核酸分子形成多種三維結構,成為適配體與靶物質特定區域結合的基礎,二者之間的結合主要通過“假堿基對”的堆積作用、氫鍵作用、靜電作用和形狀匹配等產生高特異性的結合力。適配體具有高特異性、靶分子廣、易于體外合成和修飾等優點,已經在基礎研究、臨床診斷和治療中顯示了廣闊的應用前景。
適配體的體外篩選過程稱為指數富集配體系統進化技術 (Selective expansion of ligends by exponential enrichment,SELEX),主要是模擬自然進化人工篩選技術。首先體外化學合成一個隨機堿基數為n的單鏈寡核苷酸文庫,該文庫則含4n個不同的寡核苷酸序列,常用的寡核苷酸隨機序列含30個堿基,庫容量高達430 (1018)。隨機序列的兩端是隨后PCR循環時結合引物所必需的固定序列,由于這種隨機序列,而決定了庫中每條鏈自然形成的空間構象,即二級結構的多樣性,決定了庫中潛在地存在能與各種蛋白和低分子靶物質有親和力的核酸配體。一般篩選文庫的容量巨大 (可達1015左右),理論上應用SELEX技術能篩選到自然界幾乎所有靶分子的適配子。篩選過程包含和達爾文進化理論一樣的3個過程,分別是自發突變、自然選擇、大量增殖。一般包括幾輪篩選,每輪循環包括3個主要步驟:
(1)寡核苷酸庫和靶標分子孵育;
(2)寡核苷酸復合物和未結合的寡核苷酸分離;
(3)結合的序列用PCR擴增,再進入下輪的篩選過程。
想了解更多的韓國SD產品及服務請掃描下方二維碼:我司還提供其它進口或國產試劑盒:登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團菌等試劑盒以及日本生研細菌分型診斷血清、德國SiFin診斷血清、丹麥SSI診斷血清等產品。
二維碼掃一掃
【公司名稱】 廣州健侖生物科技有限公司
【】 楊永漢
【】
【騰訊 】
【公司地址】 廣州清華科技園創新基地番禺石樓鎮創啟路63號二期2幢101-3室
【企業文化宣傳】
Therefore, the nucleic acid library can be optimized by shortening the primer sequence or eliminating the primer sequence. In the double-RNAi screening method, the primer sequences of the designed nucleic acid libraries can fold and complement themselves, forming a double-stranded structure in the primer region, so that the non-specific lattice combination of the primer sequences and target molecules may be greatly reduced. Aptamer is an oligonucleotide sequence (RNA or DNA) obtained by in vitro screening and has a strict recognition capacity and high affinity with the corresponding ligand. The size is generally about 6-40 kDa. Some secondary structures of single-stranded oligonucleotides, especially RNA, such as hairpins, stem loops, pseudoknots, convex rings, G-tetramers and the like, enable the nucleic acid molecules to form various three-dimensional structures and become aptamers The basis of binding to a specific region of the target substance, the binding between the two mainly through the "pseudo base pair" stacking effect, hydrogen bonding, electrostatic interaction and shape matching to produce high specific binding force. Aptamers have the advantages of high specificity, wide target molecules, easy synthesis and modification in vitro, and have shown broad application prospects in basic research, clinical diagnosis and treatment.
The in vitro screening of aptamers is called Selective expansion of ligends by exponential enrichment (SELEX), and is mainly based on artificial evolutionary artificial screening techniques. First, a chemically synthesized single-stranded oligonucleotide library with a random number of nucleotides n, which contains 4n different oligonucleotide sequences. The commonly used oligonucleotide random sequence contains 30 bases. The library capacity Up to 430 (1018). At both ends of the random sequence is the fixed sequence necessary for the subsequent primer binding to the primer during the PCR cycle. Due to this random sequence, the spatial conformation naturally formed by each strand in the library, ie the diversity of the secondary structure, There are potentially nucleic acid ligands that have affinity for various proteins and low molecular weight target substances. The general screening library has a huge capacity (up to about 1015). In theory, aptamers of almost all target molecules in nature can be screened by SELEX technology. The screening process contains the same three processes as Darwin's theory of evolution, namely spontaneous mutation, natural selection and mass proliferation. Generally includes several rounds of screening, each cycle consists of three main steps:
(1) oligonucleotide pool and target molecule incubation;
(2) separation of the oligonucleotide complex from the unbound oligonucleotide;
(3) The combined sequences are amplified by PCR and then into the next round of screening.
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