　　上海懋康生物科技有限公司是一家致力于生命科學和生物技術領域的高科技企業。公司由在國內科研試劑領域有著十幾年從業經驗的專業技術團隊和企業管理團隊組建而成，專門從事以抗體、細胞因子、免疫檢測試劑盒等免疫學產品為主的生物試劑的研發與銷售。公司代理40余家歐美著名生物技術公司的相關產品，能為生命科學多研究領域的廣大科研人員提供數萬種抗體、數千種蛋白以及相關檢測試劑盒等。
　　
　　上海懋康生物科技有限公司是一家涉足于生命科學和生物技術領域研究的試劑、儀器和實驗室消耗品與實驗服務工作，主要從事細胞生物學、植物學、分子生物學、免疫學、生物化學、蛋白組學。生物制藥與診斷試劑研發生產等領域。本公司秉承“以人為本，以誠為信、合同守信”的經營理念。堅持"品質保障"的原則為廣大客戶提供優質產品。

展開顯示更多內容

特美汀，金擔子素A ，潮霉素B，草銨膦，殺稻瘟菌素，嘌呤霉素，硫酸諾爾斯菌素，D-熒光素，雷帕霉素，獨腳金內酯，果膠酶Y-23，纖維素酶R-10，離析酶R-10，STZ,鏈脲佐菌素，

產品簡介在線詢價

氧化應激檢測用探針（深紅色）細胞染色氧化應激（Oxidative stress）的產生，起因于反應氧物質（ROS）生成與細胞清除ROS之間的不平衡。ROS在幾種疾病的發展中發揮重要作用，包括炎癥、動脈粥樣硬化、衰老和衰老相關的退行性疾病。

氧化應激檢測用探針（深紅色）細胞染色產品信息

CellROX Deep Red Reagent, for oxidative stress detection

氧化應激檢測用探針（深紅色）

溫馨提示：

見我司（懋康生物）整理的活性氧ROS熒光探針專題。

見我司（懋康生物）整理的CellROX Reagent, for oxidative stress detection氧化應激檢測探針產品專題。

訂購信息：

貨號	產品名稱	Ex/Em(nm)	規格	te價（元）
C10422	CellROX Deep Red Reagent 深紅色	640/665	50µl	2290

背景介紹：

氧化應激（Oxidative stress）的產生，起因于反應氧物質（ROS）生成與細胞清除ROS之間的不平衡。ROS在幾種疾病的發展中發揮重要作用，包括炎癥、動脈粥樣硬化、衰老和衰老相關的退行性疾病。

基本介紹：

CellROX Deep Red Reagent是一種檢測活細胞氧化應激的新型熒光探針。此細胞膜滲透性的染料在還原態時無熒光或呈微弱熒光，一旦被活性氧物質（ROS）氧化后發明亮穩定的熒光，最大吸收/發射波長約~ 644/ 665nm（見圖I.CellROX Deep Red氧化后的熒光光譜圖）。其熒光信號主要定位在細胞質。

CellROX Deep Red Reagent的深紅色熒光信號與其他活細胞染料和GFP兼容，使其適用于多重熒光試驗來測定各種細胞現象，包括與細胞毒性和細胞死亡的參數。不同于其它的ROS探針，比如H2DCFDA（MX4802-50MG），CellROX Deep Red Reagent的熒光信號經醛類固定后也能保留，使其相對于傳統的ROS探針來說在應用和操作流程更加靈活。CellROX Deep Red Reagent與各種檢測平臺兼容，比如傳統熒光顯微鏡、高內涵篩選儀器（HCS）、流式細胞儀、熒光酶標儀或高通量篩選（HTS）。還與各種臺式儀器兼容，比如Attune™儀器。

產品參數

外觀：無色溶液（Colorless solution）
溶劑：DMSO
激發/發射光譜范圍：644/665nm（可見光/可見光）
亞細胞定位：細胞質
熒光顏色：遠紅（Far-red）
保存方法：≤-20°C避光干燥

染色示例

氧化應激檢測用探針（深紅色）細胞染色

Fig1. BPAE detection of oxidative stress using MitoTracker® Green (Cat. No. M7514) and CellROX® Deep Red Reagent (Cat. No. C10422) BPAE cells were plated in a 96 well plate, treated with 100 µM menadione for 1 hr and then stained with 20 nM MitoTracker® Green, 5 µM Far Red ROS Sensor and Hoechst 33342 for 30 mins in complete medium. Cells were then washed 3X with PBS and imaged on a Thermo Fisher Cellomics ArrayScan® VTI. The control sample has no signal while in menadione treated cells, there is a robust increase in the signal as a result of oxidative stress caused by menadione. Both MitoTracker® Green and Far Red ROS Sensor stained well showing that they work together in a multiplex assay.

Fig2. A549 spheroid mounted with Prolong Glass and imaged with EVOS FL Auto 2. A549 spheroid grown in Nunclon Sphera microplates (Cat. No. 174925) at 1000 cells/well. Live cells were labeled with NucBlue Live ReadyProbes Reagent (Cat. No. R37605), MitoTracker Orange (Cat. No. M7510), and CellROX Deep Red Reagent for oxidative stress detection (Cat. No. C10422) for 1 hour at 37°C. Cells were then fixed with 4% PFA, transferred to a #1.5 coverslip, and mounted in ProLong Glass. Sample was allowed to cure overnight exposed to air, and the coverslip was mounted to the slide using 100% glycerol. Image acquired with EVOS FL Auto 2 (AMAFD2000) using an Olympus 10X air objective (AMEP4753).

常見問題

1）I am trying to label my cells with a reactive oxygen species (ROS) indicator dye, but I am not seeing a significant difference in signal. What could be happening?

First, make sure you have both a negative (untreated) and positive (ROS-induced) sample to compare. A good positive control can be the use of 100 μM menadione for one hour or 50 μM nefazodone for 24 hours. H2O2 can also be used, though it does not work well for CellROX™ dyes. Some dyes, such as H2DCFDA, require esterase cleavage, so don't incubate in the presence of serum (which contains esterases that can prematurely cleave the dye). If your positive control does not show significant change compared to the negative control, try increasing the concentration and label time for the dye. Our manuals give starting recommendations. Be sure to image your live cells as soon as possible. Only two dyes (CellROX™ Green and CellROX™ Deep Red) are retained with formaldehyde fixation. Finally, make sure you are using filters and instrument settings to match the excitation and emission spectra of the dye.

2）I need a formaldehyde-fixable reactive oxygen species detection assay. Is H2DCFDA fixable?

H2DCFDA and similar derivatives are not fixable. The same goes for dihydroethidium and dihydrorhodamine. However, CellROX™ Deep Red and CellROX™ Green are retained for a limited time upon fixation with formaldehyde. CellROX™ Green may be retained upon subsequent Triton™ X-100 permeabilization. Avoid the use of any acetone or alcohol-based fixatives or fixatives that include alcohol, such as formalin.

3) What dyes can I use to detect reactive oxygen species (ROS) in my bacteria?

Many dyes that are used on mammalian cells have also been shown to be useful in bacterial cells. For example, CellROX Deep Red Reagent has been shown to work in B. subtilis (see Reference:bochum.de/netahtml/HSS/Diss/RaatschenNadja/diss.pdf). If you are interested in a particular dye, but are not sure if it will work on your bacteria, literature searches are the best way to check to see if it has been tested. If not, then it may be worth testing yourself.

注意事項

為了您的安全和健康，請穿實驗服并戴一次性手套操作。

— —Written/Edited by V. Shallan【版權歸MKBio懋康所有】

上海懋康生物科技有限公司是一家涉足于生命科學和生物技術領域研究的試劑、儀器和實驗室消耗品與實驗服務工作，主要從事細胞生物學、植物學、分子生物學、免疫學、生物化學、蛋白組學。生物制藥與診斷試劑研發生產等領域。本公司秉承“以人為本，以誠為信、合同守信”的經營理念。堅持"品質保障"的原則為廣大客戶提供優質產品。

氧化應激檢測用探針（深紅色）細胞染色 氧化應激檢測用探針（深紅色）細胞染色

關鍵詞：熒光顯微鏡酶標儀